Cryopreservation of germinal stem cells in rainbow trout: a strategy to entirely restore a genotype of interest. Anne-Sophie GOUPIL et al. Conservation NOV’AE Numéro Spécial #02 RARe 2022 54-64

Gómez E, Murillo A, Carrocera S, Pérez-Jánez JJ, Benedito JL, Martín-González D and Gimeno I (2022Fitness of calves born from in vitro produced fresh and cryopreserved embryosFront. Vet. Sci. 9:1006995. doi:10.3389/fvets.2022.1006995

Rabbit pluripotent stem cells: a promising and useful
biotechnology tool Afanassieff M. et al. HAL Id: hal-03719986 https://hal-vetagro Submitted on 11 Jul 2022 World Rabbit Science Association 12th World Rabbit Congress – November 3-5 2021 – Nantes, France, Communication BP-02, 4 pp.

The Metabolic Signature of In Vitro Produced Bovine Embryos Helps Predict Pregnancy and Birth after Embryo Transfer Isabel Gimeno et al. Metabolites 2021, 11, 484.

Insights into Species Preservation: Cryobanking of Rabbit Somatic and Pluripotent Stem Cells
L Gavin-Plagne, F Perold, P Osteil, S Voisin… – International journal of …, 2020 –

32 Bovine embryo cryopreservation in a chemically defined medium A Østergaard, L Gavin-Plagne… – Reproduction …, 2020 – CSIRO Publishing

Laparoscopic insemination method in sheep allows the use of an animal protein-free and inexpensive freezing medium L Gavin-Plagne, L Boyer, A Baudot… – Annual Conference of …, 2020 –

AQUAEXCEL2020 Deliverable D7.3 Validation of the procedures for germ stem cell isolation, cryopreservation and transplantation in normal trout and carp lines. Integration of the procedures in WP3 standards Ref. Ares(2020)1784045 – 26/03/2020

Comparison Between an Animal-Derived Product Medium and a Chemically Defined Medium for Ram Sperm Cryopreservation L Gavin-Plagne, L Commin, P Bruyere… – Biopreservation and …, 2019 –

AQUAEXCEL2020 D3.3: Booklet of cryopreservation procedures for the cryobanked species Catherine Labbé, INRA Ref. Ares(2018)4996669 – 28/09/2018

Somatic Stem Cells-STEMALPHA.Cryo3 #5617

Production of Human dental pulp cells

Production of Human dental pulp cells with a medicinal manufacturing approach, 2015. Ducret et al. Basic Research Biology.

Osteoblastic differenciation

Osteoblastic Differentiation of Wharton Jelly Biopsy Specimens and Their Mesenchymal Stromal Cells after Serum-Free Culture, 2014. Andreas A. Mueller et al. Plast. Reconstr. Surg. 134: 59e, 2014.

Mesenchymal Stem Cells

Encapsulation of Mesenchymal Stem Cells by Bioscaffolds Protects Cell Survival and Attenuates Neuroinflammatory Reaction in Injured Brain Tissue After Transplantation, 2013. Anna Sarnowska et al.
Cell Transplantation, Vol. 22, Supplement 1, pp. S67–S82, 2013

Neurogenis properties

Neurogenic Properties and a Clinical Relevance of Multipotent Stem Cells Derived from Cord Blood Samples Stored in the Biobanks, 2012. Marcin Jurga et al. STEM CELLS AND DEVELOPMENT Volume 21, Number 6, 2012.


From research...

STEM ALPHA.Cryo3 #5617

Sarah Janati Idrissi. Cryoconservation d’embryons bovins produits in vitro et biopsiés. Biologie de la
reproduction. Université de Lyon, 2022. Français. NNT : 2022LYSE1020. tel-03945737

A Chemically Defined Medium for Rabbit Embryo Cryopreservation
Pierre Bruyère, Anne Baudot, Thierry Joly, Loris Commin, Elodie Pillet, Pierre Guérin, Gérard Louis, Anne Josson-Schramme, Samuel Buff ; PLoS ONE 2013 August 2013 | Volume 8 | Issue 8 | e71547

Improved cryopreservation of in vitro-produced bovine embryos using a chemically defined freezing medium Pierre Bruyère 2012 Elsevier Inc.

Veterinary applications

Rapid cooling of rabbit embryos 2018
Magda Teixeira1,*, Loris Commin1, Lucie Gavin-Plagne1, Pierre Bruyère1, Samuel Buff15 , Thierry

Embryo cryopreservation media usually contain animal-derived products, such as bovine serum albumin (BSA). These products present two major disadvantages: an undefined variable composition and a risk of pathogen transmission. We aimed to evaluate the effect of replacing BSA in rabbit embryo rapid cooling “freezing” and warming media with a chemically defined medium with no animal-derived products: STEM ALPHA.Cryo3 (“Cryo3”). A total of 1540 rabbit morulae were divided into three cryopreservation groups (group 1: BSA, group 2: 20% Cryo3 and group 3: 100% Cryo3) and a fresh controls group. After rapid cooling, embryos were cultured (in vitro approach), or transferred into synchronized does (in vivo approach). In the in vitro approach, post-warm survival rates obtained with 100% Cryo3 (94.9 %) were superior to BSA (90.8%) and 20% Cryo3 (85.6 %). The blastocyst formation rate was similar between BSA, 20% Cryo3 and 100% Cryo3 groups (85.1, 77.9 and 83.3 %, respectively), as was the expansion / hatching rate (63.1, 63.4 and 58.0%, respectively) and embryo mitochondrial activity. In the in vivo approach, pregnancy (80.0, 68.0 and 95.2 %, respectively), implantation (40.5, 45.9 and 44.8%, respectively), and live-foetus rates (35.6, 35.5 and 38.1 %, respectively) were similar between the three groups. To conclude, Cryo3 can replace BSA in rabbit embryo rapid cooling “freezing” and warming media.

Ram Sperm Cryopreservation 2018

Comparison Between an Animal-Derived Product Medium and a Chemically Defined Medium for Ram Sperm Cryopreservation

Lucie Gavin-Plagne,1,* Loris Commin,1,* Pierre Bruye` re,1 Samuel Buff,1 and Thierry Joly2

Animal-derived products are widely used in sperm cryopreservation for their cryoprotective properties. These
components, however, tend to be replaced because of sanitary risks. STEMALPHA.CRYO3 (Ref. 5617; Stem
Alpha, Saint-Genis-l’Argentie`re, France), called ‘‘CRYO3,’’ is a chemically defined preservation medium
currently used for freezing human tissue and adult stem cells. The aim of this study was to evaluate the effect of
a CRYO3-based medium on ram sperm freezing regarding in vitro parameters and in vivo fertility. Semen from
nine Charolais rams was collected using an artificial vagina, then split and frozen using two media: a CRYO3-
based medium or a control medium containing egg yolk (10%) and milk (45%). Sperm membrane integrity
(propidium iodide [PI]/SYBR-14 and calcein AM/ethidium homodimer-1), acrosome integrity (FITC-PNA/PI),
and mitochondrial membrane potential ( JC-1) were assessed using flow cytometry, while functional membrane
integrity was assessed using a hypo-osmotic swelling test and motility parameters, evaluated by computerassisted
sperm analysis. Pregnancy rates, prolificacy, and the average daily weight gain (DWG) of lambs were
evaluated after performing 195 laparoscopic inseminations. The control medium showed significantly higher
results than CRYO-based medium for all in vitro parameters, except for linearity and straightness (motions
parameters). Conversely, field trials showed no significant difference between the control medium and the
CRYO3-based medium for pregnancy rates (72.2% and 67.9%, respectively), prolificacy (1.8 and 1.6, respectively),
and the DWG (0.34 and 0.35 kg/d, respectively). This preliminary study showed that CRYO3
cannot replace egg yolk and milk in freezing extenders for commercial purposes. However, as laparoscopic
inseminations allowed a 67% pregnancy rate, CRYO3-based medium remains an option for international
transport or long-term storage of genetic diversity.

STEM ALPHA.Cryo3, 2015

Caractérisation du sperme de lapin congelé en milieux synthétiques Therapeutic application.

STEM ALPHA.Cryo3 #5617

Neurogenic Properties and a Clinical Relevance of Multipotent Stem Cells Derived from Cord Blood Samples Stored in the Biobanks Marcin Jurga, Nico Forraz, Christina Basford, Gianluigi Atzeni, Andrew J. Trevelyan, Saba Habibollah, Hamad Ali, Simon A. Zwolinski, and Colin P. McGuckin. Stem Cells and Development. April 10, 2012, 21(6): 923-936. doi:10.1089/scd.2011.0224.