RABBIT PLURIPOTENT STEM CELLS: A PROMISING AND USEFUL
BIOTEHNOLOGY TOOL Afanassieff M. et al. World Rabbit Science Association
12th World Rabbit Congress – November 3-5 2021 – Nantes, France, Communication BP-02, 4 pp. HAL Id: hal-03719986
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The Metabolic Signature of In Vitro Produced Bovine Embryos Helps Predict Pregnancy and Birth after Embryo Transfer Isabel Gimeno et al. Metabolites 2021, 11, 484. https://doi.org/10.3390/metabo11080484
Insights into Species Preservation: Cryobanking of Rabbit Somatic and Pluripotent Stem Cells
L Gavin-Plagne, F Perold, P Osteil, S Voisin… – International journal of …, 2020 – mdpi.com
32 Bovine embryo cryopreservation in a chemically defined medium A Østergaard, L Gavin-Plagne… – Reproduction …, 2020 – CSIRO Publishing
Laparoscopic insemination method in sheep allows the use of an animal protein-free and inexpensive freezing medium L Gavin-Plagne, L Boyer, A Baudot… – Annual Conference of …, 2020 – researchgate.net
Comparison Between an Animal-Derived Product Medium and a Chemically Defined Medium for Ram Sperm Cryopreservation L Gavin-Plagne, L Commin, P Bruyere… – Biopreservation and …, 2019 – liebertpub.com
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De la recherche...
STEM ALPHA.Cryo3 #5617
A Chemically Defined Medium for Rabbit Embryo Cryopreservation
Pierre Bruyère, Anne Baudot, Thierry Joly, Loris Commin, Elodie Pillet, Pierre Guérin, Gérard Louis, Anne Josson-Schramme, Samuel Buff ; PLoS ONE 2013 August 2013 | Volume 8 | Issue 8 | e71547
Improved cryopreservation of in vitro-produced bovine embryos using a chemically defined freezing medium Pierre Bruyère 2012 Elsevier Inc.
Rapid cooling of rabbit embryos 2018
Magda Teixeira1,*, Loris Commin1, Lucie Gavin-Plagne1, Pierre Bruyère1, Samuel Buff15 , Thierry
12 Embryo cryopreservation media usually contain animal-derived products, such as bovine serum
13 albumin (BSA). These products present two major disadvantages: an undefined variable
14 composition and a risk of pathogen transmission. We aimed to evaluate the effect of replacing BSA
15 in rabbit embryo rapid cooling “freezing” and warming media with a chemically defined medium with
16 no animal-derived products: STEM ALPHA.Cryo3 (“Cryo3”).
17 A total of 1540 rabbit morulae were divided into three cryopreservation groups (group 1: BSA,
18 group 2: 20% Cryo3 and group 3: 100% Cryo3) and a fresh controls group. After rapid cooling,
19 embryos were cultured (in vitro approach), or transferred into synchronized does (in vivo
20 approach). In the in vitro approach, post-warm survival rates obtained with 100% Cryo3 (94.9 %)
21 were superior to BSA (90.8%) and 20% Cryo3 (85.6 %). The blastocyst formation rate was similar
22 between BSA, 20% Cryo3 and 100% Cryo3 groups (85.1, 77.9 and 83.3 %, respectively), as was
23 the expansion / hatching rate (63.1, 63.4 and 58.0%, respectively) and embryo mitochondrial
24 activity. In the in vivo approach, pregnancy (80.0, 68.0 and 95.2 %, respectively), implantation
25 (40.5, 45.9 and 44.8%, respectively), and live-foetus rates (35.6, 35.5 and 38.1 %, respectively)
26 were similar between the three groups. To conclude, Cryo3 can replace BSA in rabbit embryo rapid
27 cooling “freezing” and warming media.
Ram Sperm Cryopreservation 2018
Comparison Between an Animal-Derived Product Medium and a Chemically Defined Medium for Ram Sperm Cryopreservation
Lucie Gavin-Plagne,1,* Loris Commin,1,* Pierre Bruye` re,1 Samuel Buff,1 and Thierry Joly2
Animal-derived products are widely used in sperm cryopreservation for their cryoprotective properties. These
components, however, tend to be replaced because of sanitary risks. STEMALPHA.CRYO3 (Ref. 5617; Stem
Alpha, Saint-Genis-l’Argentie`re, France), called ‘‘CRYO3,’’ is a chemically defined preservation medium
currently used for freezing human tissue and adult stem cells. The aim of this study was to evaluate the effect of
a CRYO3-based medium on ram sperm freezing regarding in vitro parameters and in vivo fertility. Semen from
nine Charolais rams was collected using an artificial vagina, then split and frozen using two media: a CRYO3-
based medium or a control medium containing egg yolk (10%) and milk (45%). Sperm membrane integrity
(propidium iodide [PI]/SYBR-14 and calcein AM/ethidium homodimer-1), acrosome integrity (FITC-PNA/PI),
and mitochondrial membrane potential ( JC-1) were assessed using flow cytometry, while functional membrane
integrity was assessed using a hypo-osmotic swelling test and motility parameters, evaluated by computerassisted
sperm analysis. Pregnancy rates, prolificacy, and the average daily weight gain (DWG) of lambs were
evaluated after performing 195 laparoscopic inseminations. The control medium showed significantly higher
results than CRYO-based medium for all in vitro parameters, except for linearity and straightness (motions
parameters). Conversely, field trials showed no significant difference between the control medium and the
CRYO3-based medium for pregnancy rates (72.2% and 67.9%, respectively), prolificacy (1.8 and 1.6, respectively),
and the DWG (0.34 and 0.35 kg/d, respectively). This preliminary study showed that CRYO3
cannot replace egg yolk and milk in freezing extenders for commercial purposes. However, as laparoscopic
inseminations allowed a 67% pregnancy rate, CRYO3-based medium remains an option for international
transport or long-term storage of genetic diversity.
STEM ALPHA.Cryo3, 2015
Caractérisation du sperme de lapin congelé en milieux synthétiques
...vers un usage thérapeutique.
STEM ALPHA.Cryo3 #5617
Neurogenic Properties and a Clinical Relevance of Multipotent Stem Cells Derived from Cord Blood Samples Stored in the Biobanks Marcin Jurga, Nico Forraz, Christina Basford, Gianluigi Atzeni, Andrew J. Trevelyan, Saba Habibollah, Hamad Ali, Simon A. Zwolinski, and Colin P. McGuckin. Stem Cells and Development. April 10, 2012, 21(6): 923-936. doi:10.1089/scd.2011.0224.