Gómez E, Murillo A, Carrocera S, Pérez-Jánez JJ, Benedito JL, Martín-González D and Gimeno I (2022) Fitness of calves born from in vitro produced fresh and cryopreserved embryos. Front. Vet. Sci. 9:1006995. doi:10.3389/fvets.2022.1006995
RABBIT PLURIPOTENT STEM CELLS: A PROMISING AND USEFUL
BIOTEHNOLOGY TOOL Afanassieff M. et al. World Rabbit Science Association
12th World Rabbit Congress – November 3-5 2021 – Nantes, France, Communication BP-02, 4 pp. HAL Id: hal-03719986
Submitted on 11 Jul 2022
The Metabolic Signature of In Vitro Produced Bovine Embryos Helps Predict Pregnancy and Birth after Embryo Transfer Isabel Gimeno et al. Metabolites 2021, 11, 484. https://doi.org/10.3390/metabo11080484
Insights into Species Preservation: Cryobanking of Rabbit Somatic and Pluripotent Stem Cells
L Gavin-Plagne, F Perold, P Osteil, S Voisin… – International journal of …, 2020 – mdpi.com
32 Bovine embryo cryopreservation in a chemically defined medium A Østergaard, L Gavin-Plagne… – Reproduction …, 2020 – CSIRO Publishing
Laparoscopic insemination method in sheep allows the use of an animal protein-free and inexpensive freezing medium L Gavin-Plagne, L Boyer, A Baudot… – Annual Conference of …, 2020 – researchgate.net
Comparison Between an Animal-Derived Product Medium and a Chemically Defined Medium for Ram Sperm Cryopreservation L Gavin-Plagne, L Commin, P Bruyere… – Biopreservation and …, 2019 – liebertpub.com
Cellules souches somatiques
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Mesenchymal Stem Cells
Encapsulation of Mesenchymal Stem Cells by Bioscaffolds Protects Cell Survival and Attenuates Neuroinflammatory Reaction in Injured Brain Tissue After Transplantation, 2013. Anna Sarnowska et al.
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De la recherche...
STEM ALPHA.Cryo3 #5617
Sarah Janati Idrissi. Cryoconservation d’embryons bovins produits in vitro et biopsiés. Biologie de la
reproduction. Université de Lyon, 2022. Français. NNT : 2022LYSE1020. tel-03945737 https://theses.hal.science/tel-03945737
A Chemically Defined Medium for Rabbit Embryo Cryopreservation
Pierre Bruyère, Anne Baudot, Thierry Joly, Loris Commin, Elodie Pillet, Pierre Guérin, Gérard Louis, Anne Josson-Schramme, Samuel Buff ; PLoS ONE 2013 August 2013 | Volume 8 | Issue 8 | e71547
Improved cryopreservation of in vitro-produced bovine embryos using a chemically defined freezing medium Pierre Bruyère 2012 Elsevier Inc.
Rapid cooling of rabbit embryos 2018
Magda Teixeira1,*, Loris Commin1, Lucie Gavin-Plagne1, Pierre Bruyère1, Samuel Buff15 , Thierry
Embryo cryopreservation media usually contain animal-derived products, such as bovine serum albumin (BSA). These products present two major disadvantages: an undefined variable composition and a risk of pathogen transmission. We aimed to evaluate the effect of replacing BSA in rabbit embryo rapid cooling “freezing” and warming media with a chemically defined medium with no animal-derived products: STEM ALPHA.Cryo3 (“Cryo3”). A total of 1540 rabbit morulae were divided into three cryopreservation groups (group 1: BSA, group 2: 20% Cryo3 and group 3: 100% Cryo3) and a fresh controls group. After rapid cooling, embryos were cultured (in vitro approach), or transferred into synchronized does (in vivo approach). In the in vitro approach, post-warm survival rates obtained with 100% Cryo3 (94.9 %) were superior to BSA (90.8%) and 20% Cryo3 (85.6 %). The blastocyst formation rate was similar between BSA, 20% Cryo3 and 100% Cryo3 groups (85.1, 77.9 and 83.3 %, respectively), as was the expansion / hatching rate (63.1, 63.4 and 58.0%, respectively) and embryo mitochondrial activity. In the in vivo approach, pregnancy (80.0, 68.0 and 95.2 %, respectively), implantation (40.5, 45.9 and 44.8%, respectively), and live-foetus rates (35.6, 35.5 and 38.1 %, respectively) were similar between the three groups. To conclude, Cryo3 can replace BSA in rabbit embryo rapid cooling “freezing” and warming media.
Ram Sperm Cryopreservation 2018
Comparison Between an Animal-Derived Product Medium and a Chemically Defined Medium for Ram Sperm Cryopreservation
Lucie Gavin-Plagne,1,* Loris Commin,1,* Pierre Bruye` re,1 Samuel Buff,1 and Thierry Joly2
Animal-derived products are widely used in sperm cryopreservation for their cryoprotective properties. These components, however, tend to be replaced because of sanitary risks. STEMALPHA.CRYO3 (Ref. 5617; Stem Alpha, Saint-Genis-l’Argentiere, France), called ‘‘CRYO3,’’ is a chemically defined preservation medium
currently used for freezing human tissue and adult stem cells. The aim of this study was to evaluate the effect of a CRYO3-based medium on ram sperm freezing regarding in vitro parameters and in vivo fertility. Semen from nine Charolais rams was collected using an artificial vagina, then split and frozen using two media: a CRYO3- based medium or a control medium containing egg yolk (10%) and milk (45%). Sperm membrane integrity (propidium iodide [PI]/SYBR-14 and calcein AM/ethidium homodimer-1), acrosome integrity (FITC-PNA/PI), and mitochondrial membrane potential ( JC-1) were assessed using flow cytometry, while functional membrane integrity was assessed using a hypo-osmotic swelling test and motility parameters, evaluated by computerassisted sperm analysis. Pregnancy rates, prolificacy, and the average daily weight gain (DWG) of lambs were evaluated after performing 195 laparoscopic inseminations. The control medium showed significantly higher results than CRYO-based medium for all in vitro parameters, except for linearity and straightness (motions parameters). Conversely, field trials showed no significant difference between the control medium and the CRYO3-based medium for pregnancy rates (72.2% and 67.9%, respectively), prolificacy (1.8 and 1.6, respectively), and the DWG (0.34 and 0.35 kg/d, respectively). This preliminary study showed that CRYO3 cannot replace egg yolk and milk in freezing extenders for commercial purposes. However, as laparoscopic inseminations allowed a 67% pregnancy rate, CRYO3-based medium remains an option for international transport or long-term storage of genetic diversity.
STEM ALPHA.Cryo3, 2015
Caractérisation du sperme de lapin congelé en milieux synthétiques
...vers un usage thérapeutique.
STEM ALPHA.Cryo3 #5617
Neurogenic Properties and a Clinical Relevance of Multipotent Stem Cells Derived from Cord Blood Samples Stored in the Biobanks Marcin Jurga, Nico Forraz, Christina Basford, Gianluigi Atzeni, Andrew J. Trevelyan, Saba Habibollah, Hamad Ali, Simon A. Zwolinski, and Colin P. McGuckin. Stem Cells and Development. April 10, 2012, 21(6): 923-936. doi:10.1089/scd.2011.0224.