Bogdan Spiru et al, Cornea 2017

Bogdan Spiru et al, Cornea 2017


Purpose: To evaluate the biomechanical stability of ex vivo porcine corneas after femtosecond lenticule extraction (FLEx) and small incision lenticule extraction (SmILE) refractive surgeries.

Methods: Forty-five porcine eyes were equally divided into three groups: Groups 1 and 2 were treated with FLEx and SmILE procedure, respectively. Group 3 served as control. A refractive correction of −14 diopters (D) with a 7-mm zone using either a 160-μm flap (FLEx) or a 160-μm cap (SmILE) was performed. For two-dimensional (2D) elastic and viscoelastic biomechanical characterization, two testing cycles (preconditioning stress–strain curve from 1.27 to 12.5 N, stress–relaxation at 12.5 N during 120 seconds) were conducted. Young's modulus and Prony constants were calculated.

Results: At 0.8% of strain, FLEx (370 ± 36 kPa) could resist a significantly lower stress than SmILE (392 ± 19 kPa, P = 0.046) and the control group (402 ± 30 kPa, P = 0.013). Also, FLEx (46.1 ± 4.5 MPa) had a significantly lower Young's modulus than the control group (50.2 ± 3.4 MPa, P = 0.008). The Young's modulus of SmILE (48.6 ± 2.5 MPa) had values situated between untreated corneas and FLEx-treated corneas. When compared to untreated controls, the stress resistance decreased by 8.0% with FLEx and 2.5% with SmILE; Young's modulus decreased by 5.1% with FLEx and 1.04% with SmILE. With a cap-based procedure, both anterior cap and stromal bed carry the intraocular pressure, while in a flap-based procedure, only the stromal bed does.

Conclusions: Compared to flap-based procedures like FLEx, the cap-based technique SmILE can be considered superior in terms of biomechanical stability, when measured experimentally in ex vivo porcine corneas. 

Marie Regnier et al. Medicine 2017

Marie Regnier et al. Medicine 2017

Eye bank prepared versus surgeon cut endothelial graft tissue for Descemet membrane endothelial keratoplasty: An observational study.
Regnier, Marie MD a; Auxenfans, Celine PhD b; Maucort-Boulch, Delphine MD, PhD c,d,e; Marty, Anne-Sophie MD a; Damour, Odile PhD b; Burillon, Carole MD a,b,c; Kocaba, Viridiana MD a,b,c,*
[Article] Medicine. 96(19):e6885, May 2017. 


The purpose of this article is to examine outcomes of Descemet membrane endothelial keratoplasty (DMEK) performed with cornea bank (CB) prestripped tissue and surgeon stripped tissue (SST).

This retrospective study examined subjects who underwent DMEK with CB or surgeon prepared tissue for Fuchs endothelial corneal dystrophy. Best-corrected visual acuity (BCVA), corneal thickness, endothelial cell count (ECC), and complications were examined before and throughout a 6-month postoperative period.

Eleven CB and 22 SST subjects were included. Six months after surgery, BCVA was 20/20 or better in 36.4% of CB and 22.7% of SST subjects (P = .43). Median logMAR BCVA was 0.10 (0.00-0.20, 20/25) in group CB and 0.10 (0.10-0.30, 20/25) in group SST. Median preoperative corneal thickness was 614.0 [mu]m (577.5-662.0 [mu]m) and 658.0 [mu]m (606.0-689.0 [mu]m) in CB and SST subjects, respectively (P = .37). Six months after surgery, median corneal thickness was lower in the CB group (571.0 [mu]m [478.0-592.0 [mu]m]), than in the SST group (576.0 [mu]m [531.0-607.0 [mu]m], P = .02). At 6 months, median ECC was 1500.0 cell/mm2 (1321.5-2049.0 cell/mm2, 41% decrease) in group CB and 1403.0 cell/mm2 (972.5-2010.7 cell/mm2, 46% decrease) in group SST (P = .70). Rebubbling was required in 5 CB (45.5%) and 15 SST (68.2%) subjects (P = .39).

Fuchs' dystrophy patients have good anatomic and functional DMEK results. Similar outcomes and complication rates occurred with eye bank and surgeon prepared donor tissue.


An-Katrien De Roo et al, ivos 2017

An-Katrien De Roo et al, ivos 2017

Identification of Circulating Fibrocytes and Dendritic Derivatives in Corneal Endothelium of Patients With Fuchs’ Dystrophy

R Hristova et al, 2016


ЛИМБАЛНА БИОПСИЯ След получаване на информирано съгласие бе проведена минимално инвазивна лимбална биопсия. Процедурата включва внимателна дисекция на лимбален епител с размер 2х2 мм в зона с демонстративни палисади на Vogt на 12 ч. при стриктно спазване на асептични условия. В два от случаите за донор бе използвано контралатералното здраво око. При другите двама пациенти биопсията бе получена от зона със запазени палисади на засегнатото око. Полученият материал бе транспортиран до тъканна банка Биорегенерация в среда Stem α 2 (STEM ALPHA, Rhône-Alpes, France).



R. Hristova, Y. Zdravkov, M. Hristova, I. Tanev


The aim of the current study is to investigate the application of autologous ex vivo expanded limbal stem cell transplantation in the management of ocular surface disease. This is the fi rst study in Bulagria in which autologous ex vivo expanded stem cells were transplanted to four patients with mean age 56.5 years from the Department of Ophthalmology, University Hospital Alexandrovska. All patients presented signs of unilateral limbal stem cell defi ciency, associated with trophic disease of the ocular surface. After informed consent was obtained a minimally invasive limbal biopsy was performed. The limbal materials were cultured using a novel protocol without additional xenobiotic products. The grafts were transplanted after fourteen days. Anterior segment optical coherence tomography before and after the intervention was used for assessment of the condition. Success of the procedure was defi ned as 1  . complete epithelialization of the corneal surface, 2. partial or complete reduction of neovascularization, 3. achieving normal corneal transparency, thickness and refl ex, 4. visual acuity improvement. Clinical recovery was observed in all patients, as well as improvement of subjective ocular comfort. In three of the cases all four success criteria were met. Visual acuity of one patient did not change, due to neoplastic process, pervading the optic nerve. No graft rejection reactions were observed. Limbal biopsy did not induce limbal stem cell defi ciency in the donor eye. Transplantation of autologous ex vivo expanded limbal stem cells is an effective and safe method for ocular surface reconstruction, which can be applied in different conditions, associated with limbal stem cell defi ciency.


Contact address: Rozaliya Hristova, 1 G. Sofi iyski str. Sofi a 1431 е-mail:

G. Ho Wang Yin_J Fr Ophtalmol_2016

G. Ho Wang Yin_J Fr Ophtalmol_2016

Impact des caractéristiques du greffon cornéen sur les résultats cliniques après Descemet stripping automated endothelial keratoplasty (DASEK).


Effect of donor graft characteristics on clinical outcomes in Descemet stripping automated endothelial keratoplasty (DASEK). 



Simultaneous microstructural and mechanical characterization of human corneas at increasing pressure. JMBBM 2016

Milieux de conservation : Cornée Humaine

In vivo structural imaging of the cornea by polarization-resolved second harmonic microscopy

Gaël Latour,1 Ivan Gusachenko,1 Laura Kowalczuk,2 Isabelle Lamarre,1 and Marie‑Claire Schanne-Klein1,*

1Laboratory for Optics and Biosciences, École Polytechnique—CNRS—INSERM, 91128 Palaiseau, France

2Laboratory for Applied Optics, ENSTA ParisTech—École Polytechnique—CNRS, 91761 Palaiseau, France *Corresponding author:


Received 7 Nov 2011; revised 29 Nov 2011; accepted 29 Nov 2011; published 1 Dec 2011

(C) 2011 OSA 1 January 2012 / Vol. 3, No. 1 / BIOMEDICAL OPTICS EXPRESS 1


Gain P., Dumollard JM, Peoc’h M, Thuret G. Limite du modèle de greffe de cornée chez le lapin pour évaluer la conservation en organoculture dans un nouveau milieu sans composant animal et la déturgescence en poloxamer, 112è Congrès de la Société Française d’Ophtalmologie, 29, Hors série 1, 2006.

Thuret G., Manissolle C., Campos-Guyotat L, Guyotat D and Gain P. (2005) Animal compound free medium and poloxamer for human corneal organ culture and deswelling, Investigative Ophthalmology & Visual Science, 46, 816-822.

G. Thuret, C. Manissolle, O. Garraud, S. Acquart, L. Campos, D. Guyotat, J. Maugery and P. Gain. New animal compound free medium for human corneal organ culture, Invest Ophthalmol Vis Sci 2004 ; 45 : E-Abstract 4868.


Milieux de conservation : Cornée Animale

Cornées de lapin, de rat et de cochon : caractéristiques principales etconservation en organo-cultureE. Crouzet, Z. He, S. Piselli, N. Campolmi, F. Forest, M. Peoc'H, JM. Dumollard, P. Gain, G. Thuret (SFO 2013)



A l’exception des primates, les modèles cornéens animaux sont éloignés de l’homme. Les modèles cornéensanimaux de conservation sont virtuellement inexistants. Le but de ce travail était d’établir les principalescaractéristiques, en particulier celles concernant les cellules endothéliales (CE), de la cornée de 3 animaux delaboratoire facilement disponibles, ainsi que leurs capacités à être conservée en organoculture (OC)


Trente cornées de cochons Large White âgés de 6 mois, 20 de lapin California âgés de 10 semaines et 10 derats Lewis âgés de 8 semaines ont été analysées. Données macroscopiques : 1/diamètre vertical et horizontal(compas chirurgical) 2/ transparence (analyse de la fonction de modulation de transfert), 3/ épaisseur centrocornéenne (pachymètre à ultrasons). Données microscopiques : 1/ histologie sur coupes colorées àl’Hématéine-Eosine-Safranine et ultrastructure, 2/ Densité cellulaire endothéliale (DCE) et morphométrie, 3/statut prolifératif des CE (Ki67 et incorporation de 5-Ethynyl-2’-Deoxyuridine (Clik-it EdU)), état dedifférenciation (Na+/K+ ATPase, ZO-1, JAM-1), et existence de cellules souches (Nestin, ABCG2,Télomérase). Les cornées fraîches ont été placées en OC dans deux milieux commercialisés (CorneaMax,Eurobio, Les Ulis, France ; et StemAlpha, St Genis L'Argentière, France). L’épaisseur a été mesurée toutes les2h durant 6h, puis toutes les 12h. La transparence et la viabilité des CE (1) ont été déterminées après 2, 3 et4 jours d’OC


Pour les trois espèces animales, DCE, polymorphisme et polymégéthisme étaient plus élevés que chezl’homme. L’épaisseur augmentait très rapidement dans les deux milieux de cultures, aboutissant à des tissusbiconvexes avec perte quasi totale de la transparence cornéenne et plissement significatif de l’endothélium.La viabilité des CE diminuait surtout dans les plis


Nous avons pu constituer une base de données complète des caractéristiques cornéennes ex-vivo de troisespèces : rat, cochon et lapin


Les cornées des trois animaux ne peuvent pas être placées plus de 2 à 3 jours dans un milieu d’OC fabriquéinitialement pour des cornées humaines. Des milieux spécifiques doivent être développés pour obtenir desmodèles animaux fiables en OC.Mots-clés :Kératoplastie transfixiante