A Bourdieu et al, J Cell Physiol 2017

Steady state peripheral blood provides cells with functional and metabolic characteristics of real hematopoietic stem cells.

J Cell Physiol.  2017;  (ISSN: 1097-4652)

Bourdieu A; Avalon M; Lapostolle V; Ismail S; Mombled M; Debeissat C; Guérinet M; Duchez P; Chevaleyre J; Vlaski-Lafarge M; Villacreces A; Praloran V; Ivanovic Z; Brunet de la Grange P


Hematopoietic stem cells (HSCs), which are located in the bone marrow, also circulate in cord and peripheral blood. Despite high availability, HSCs from steady state peripheral blood (SSPB) are little known and not used for research or cell therapy. We thus aimed to characterize and select HSCs from SSPB by a direct approach with a view to delineating their main functional and metabolic properties and the mechanisms responsible for their maintenance. We chose to work on Side Population (SP) cells which are highly enriched in HSCs in mouse, human bone marrow, and cord blood. However, no SP cells from SSBP have as yet been characterized. Here we showed that SP cells from SSPB exhibited a higher proliferative capacity and generated more clonogenic progenitors than non-SP cells in vitro. Furthermore, xenotransplantation studies on immunodeficient mice demonstrated that SP cells are up to 45 times more enriched in cells with engraftment capacity than non-SP cells. From a cell regulation point of view, we showed that SP activity depended on O2 concentrations close to those found in HSC niches, an effect which is dependent on both hypoxia-induced factors HIF-1α and HIF-2α. Moreover SP cells displayed a reduced mitochondrial mass and, in particular, a lower mitochondrial activity compared to non-SP cells, while they exhibited a similar level of glucose incorporation. These results provided evidence that SP cells from SSPB displayed properties of very primitive cells and HSC, thus rendering them an interesting model for research and cell therapy.  


Product used : STEM LAPHA.1D (#5112)

Contrôle Progéniteurs Hématopoïétiques

BILAN 2009 - 2010 ET ÉVOLUTION DU CONTRÔLE EXTERNE DES CELLULES SOUCHES HEMATOPOÏETIQUES B. Panterne, C. Sabatini, V. Duval, G. Huyghe*, L. Fleury et L. Mouillot Agence francaise de sécurite sanitaire des produits de santé (Afssaps) – Direction des laboratoires et des controles (DLC) – Unité Produits sanguins et therapie cellulaire. *Unite Microbiologie, 143/147, Bd Anatole France, 93285 Saint-Denis Cedex


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Milieu liquide sans sérum STEM ALPHA.A

En 2012

Mohammad Hammoud et al. 2012. Combination of low O2 concentration and mesenchymal stromal cells during culture of cord blood CD34+ cells improves the maintenance and proliferative capacity of hematopoietic stem cells. 

Article first published online: 24 FEB 2012 DOI: 10.1002/jcp.23019

Journal of Cellular Physiology Vol 227, Issue 6, p. 2750–2758, June 2012


En 2011

Membrane-bound IL-15 stimulation on peripheral blood NK ...

de S Negrini - 2011 - Published Ahead of Print on January 17, 2011,  expanded at high cell density in STEMα−Α medium (Stem Alpha, Saint Clement les places, ...)


En 2010

Modeling of congenital erythropoietic porphyria by RNA interference: a new tool for preclinical gene therapy evaluation

Elodie Robert-Richard1,2, Magalie Lalanne1,2, Isabelle Lamrissi-Garcia1,2, Véronique Guyonnet-Duperat1, Emmanuel Richard1,2, Vincent Pitard2,3, Fréderic Mazurier1,2, François Moreau-Gaudry1,2, Cécile Ged1,2, Hubert de Verneuil1,2,*

The Journal of Gene Medicine

Volume 12, Issue 8, pages 637–646, August 2010


En 2009

Low O2 concentrations enhance the positive effect of IL-17 on the maintenance of erythroid progenitors during co-culture of CD34+ and mesenchymal stem cells

Eur. Cytokine Netw., Vol. 20 n° 1, March 2009, 10-6


Aleksandra Krstić1, Marija Vlaski2,3, Mohammad Hammoud2, Jean Chevaleyre2,

Pascale Duchez2, Gordana Jovčić1, Diana Bugarski1, Pavle Milenković1, Philippe Bourin4,Jean-Michel Boiron2,3, Vincent Praloran3,5, Zoran Ivanović2

1 Institute for Medical Research, Belgrade University, Belgrade, Serbia

2 French Blood Institute, Aquitaine-Limousin Branch, 5, place Amélie Raba Léon, BP 24, 33035 Bordeaux Cedex, France

3 Victor-Ségalen University (Bordeaux 2), Bordeaux, France

4 French Blood Institute, Pyrénées-Méditerranée Branch, Toulouse, France

5 CNRS UMR 5164 Correspondence: Z. Ivanovic, MD, PhD, Etablissement Français du Sang Aquitaine-Limousin, 5 place Amélie Raba Léon, BP 24,

33035 Bordeaux Cedex, France <zoran.ivanovic@efs.sante.fr> Accepted for publication January 16, 2009


Tissue inhibitors of matrix metalloproteinases in platelets and megakaryocytes: A novel organization for these secreted proteins

Julien Villeneuve,  Anna Block Marie-Caroline Le Bousse-Kerdilès, Sébastien Lepreux Paquita Nurden

Jean Ripoche  Alan T. Nurden

Experimental Hematology Volume 37, Issue 7 , Pages 849-856, July 2009


Review of Althought - Definition and Scientific Information - Page 1

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The peripheral CD138+ population but not the CD138) population contains myeloma clonogenic cells in plasma cell leukaemia patients 2011 Blackwell Publishing Ltd, British Journal of Haematology doi:10.1111/j.1365-2141.2011.08904.x David Chiron, Sylvanie Surget, Sophie Maı¨ga, Re´gis Bataille, , Philippe Moreau, , Steven Le Gouil, Martine Amiot,  , Catherine Pellat-Deceunynck


Leukemia 24, 601-612 (March 2010)

Functional characterization of high levels of meningioma 1 as collaborating oncogene in acute leukemia

T Liu, D Jankovic, L Brault, S Ehret, F Baty, V Stavropoulou, V Rossi, A Biondi and J Schwaller



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